Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

NEK7 Cys298 is important for NLRP3 inflammasome and targetable by dimethyl fumarate.

Cell chemical biology·2026
Same author

Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation.

Methods in enzymology·2026
Same author

Quantitative analysis of H-Ras localization using confocal microscopy.

Methods in enzymology·2026
Same author

Organotellurium Probes Enable One-step Single-cell Analysis of Post-translational Modification.

Journal of the American Chemical Society·2026
Same author

Synthetic Routes to, Stabilities and Transformations of, and Characterization of (Carbamoyl)disulfanyl Chlorides and Related Compounds<sup>1</sup><sup>,</sup><sup>2</sup>.

Molecules (Basel, Switzerland)·2025
Same author

Profiling of C-terminal prenylated proteins using tandem mass tagging.

Methods in enzymology·2025

相关实验视频

Updated: Jun 23, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

用于活细胞分析的多功能前化.

James W Wollack1, Nicholette A Zeliadt, Daniel G Mullen

  • 1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Journal of the American Chemical Society
|May 12, 2009
PubMed
概括

研究人员开发了光,以跟踪活细胞中的蛋白质先化. 这些进入细胞并定位在细胞核附近,有助于研究前化和细胞内贩运.

科学领域:

  • 生物化学 生物化学
  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 蛋白质前化是真核细胞中一个关键的翻译后修饰,调节信号转导通路.
  • 抑制蛋白质前化显示出治疗潜力,但体外研究限制了对其体内局部化的理解.
  • 研究活细胞中的先化对于理解其生物作用和开发向疗法至关重要.

研究的目的:

  • 合成和分析光标记的酸,用于研究活细胞中的蛋白质化.
  • 为了研究先化的细胞内定位和贩运.
  • 建立一种合成策略,以创建具有酸敏感功能的.

主要方法:

  • 开发一种合成途径,用于多功能,包括光体和细胞透序列 (例如,penetratin).
  • 使用了一种新型的Acm到Scm保护组转换,与对酸敏感的异oprenoid部分兼容.
  • 活细胞成像分析吸收机制和基于prenylation和penetratin存在的细胞内定位.

主要成果:

  • 基于CDC42蛋白C端的合成光,使得活细胞分析前化.
  • 证明,即使使用完整的CAAX盒,也可以独立于透的化进入细胞.
  • 观察到能量独立的细胞进入和缺乏透蛋白的先化的周核定位.

更多相关视频

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

相关实验视频

Last Updated: Jun 23, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

结论:

  • 开发的提供了一种新的工具,用于研究蛋白质前化动力学和活细胞内的定位.
  • 合成方法非常适用于组装具有酸敏感组的,扩大化学生物学研究的可能性.
  • 这些发现有助于未来研究酶处理和先化分子的细胞内贩运.