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ангиотензин转化酶:通过类固醇诱导子膜巨细胞在培养中
Science (New York, N.Y.)
|July 1, 1977
概括
像德克萨梅他松和普雷德尼松这样的葡萄糖皮质类药物显著增加了子肺细胞中的血管素转化酶. 这表明新的基因活动和蛋白质生产调节了这种酶,这对于血压控制至关重要.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- ангиотензин转化酶 (ACE) 在血压调节中起着至关重要的作用.
- 了解ACE活动的调节对于开发治疗策略很重要.
- 已知葡萄皮质类药物会影响各种细胞过程.
研究的目的:
- 为了研究葡萄糖皮质类药物对子膜巨细胞中的血管酶转化酶活性的影响.
- 确定观察到的ACE活性变化是否依赖于新的基因转录和蛋白质合成.
主要方法:
- 子膜巨细胞在体外培养.
- 细胞被用德克萨米他松和普雷迪尼松在生理学度 (4nM) 的治疗.
- 通过使用actinomycin D (转录抑制剂) 和cycloheximide (转化抑制剂) 进行了抑制研究.
- 测量了 ангиотензин转化酶的活性.
主要成果:
- 与对照人群相比,德克萨米他和普雷迪尼在3天内增加了血管激素转化酶活性7至16倍.
- 在4nM的类固醇度下观察到最大的刺激.
- 酶活性的增加被actinomycin D和cycloheximide显著抑制.
- 这些发现表明,葡萄糖皮质激素诱导的ACE升高需要新的转录和合成.
结论:
- 在生理学度下,葡萄糖皮质类药物会诱导子膜巨细胞中血管酶转化酶活性显著增加.
- 葡萄糖皮质体对血管酶转化酶的调节涉及新的转录和酶合成.
- 这项研究提供了血管酶转化酶的新型调节机制的证据,突出了其在血压控制中的潜在作用.
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