CD11c+单细胞在与高胆固醇血清症相关的动脉生成中的功能性作用
Huaizhu Wu1, R Michael Gower, Hong Wang
1Section of Atherosclerosis and Vascular Medicine, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Circulation
|May 13, 2009
概括
高胆固醇会增加单细胞上的CD11c,这是一种对它们迁移至关重要的蛋白质. 阻止CD11c可减少单细胞积累和动脉样硬化在小鼠模型中,突出其在心血管疾病中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 心血管生物学 心血管生物学
- 动脉样硬化研究 动脉样硬化研究
背景情况:
- 单细胞激活和迁移是动脉样硬化症的核心,这种疾病因高胆固醇血清症而恶化.
- β2整合素CD11c/CD18在单细胞上表达并影响它们的粘附,但其在高胆固醇血清症驱动动的动脉样硬化中的作用尚不清楚.
研究的目的:
- 为了研究高胆固醇血症中CD11c表达的调节.
- 在小鼠模型中确定CD11c在单细胞招募和动脉样硬化发展中的作用.
主要方法:
- 产生了缺乏CD11c的小鼠,并将它们与缺乏apolipoprotein E (apoE-/-) 的小鼠交配.
- 利用流式细胞计量来分析高胆固醇贫血小鼠白细胞上的CD11c表达.
- 在CD11c缺乏和对照小鼠中评估单细胞粘附,病变积累和动脉样硬化发展.
主要成果:
- 与对照组相比,高脂肪饮食的高胆固醇贫血apoE-/-小鼠表现出CD11c+单细胞增加.
- 这些CD11c+单细胞显示了脂质积累的迹象,并增加了CD11b和CD29的表达.
- 缺少CD11c减少了单细胞对内皮连接体的附着性,并减少了动脉样硬化病变的发展.
结论:
- 在高胆固醇血症期间,血液单细胞上的CD11c表达增加.
- CD11c在单细胞在动脉壁的招募中起着重要作用.
- 向CD11c可能提供一种治疗策略,以减少高胆固醇血清症中的动脉样硬化.
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