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在体内直接检测流产性RNA转录物

Seth R Goldman¹, Richard H Ebright, Bryce E Nickels

¹Department of Genetics and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA.

Science (New York, N.Y.)

|May 16, 2009

概括

堕胎启动,即RNA聚合酶 (RNAP) 过早释放短RNA,发生在细菌体内. 这一发现表明堕胎转录可能调节基因表达.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
微生物学微生物学

背景情况:

堕胎启动是一个已知的现象,在体外转录过程中,由 prokaryotic 和 eukaryotic RNA 聚合酶 (RNAP).
它涉及在生产性转录启动之前合成和释放短RNA转录 (2-15个核酸).
这种过程是否发生在生物体内仍然是未知的.

研究的目的:

为了确定堕胎启动是否发生在细菌中的体内.
描述堕胎启动的体内行为,并将其与体外观察进行比较.

主要方法:

在细菌中直接检测到堕胎的转录,使用与锁定核酸探针的杂交.
在特定的遗传和分子条件下,分析了堕胎启动频率的变化.

主要成果:

在细菌体内体内成功检测到流产性转录.
证明了体外堕胎启动与体外堕胎启动具有共同的特征.
在稳定RNAP促进DNA或RNAP-sigma因子相互作用时观察到堕胎启动的增加.
发现删除延长因子GreA也增加了体内流产的启动.

结论:

流产启动发生在细菌体内.
在体外堕胎启动受到影响的因素影响RNAP-DNA和RNAP-sigma相互作用,类似于体外.
活体中流产性转录的存在表明在基因表达调节中的潜在功能性作用.

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