在鱼物种中保存多态简单序列位点
C Schlötterer1, B Amos, D Tautz
1Institut für Genetik und Mikrobiologie, Universität München, Germany.
Nature
|November 7, 1991
概括
为试点鱼开发的简单序列长度多态 (SSLP) 起始剂成功地在各种鱼物种中放大了DNA. 这种对非编码DNA序列的高度保护简化了对鱼的遗传研究.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 海洋哺乳动物科学科学
背景情况:
- 简单序列长度多态 (SSLP) 在真核细胞DNA中很常见,对种群遗传学有价值.
- 试点 (Globicephala melas) 中的SSLP以前已经对它们的交配系统和种群结构产生了洞察力.
研究的目的:
- 为了研究聚合酶连锁反应 (PCR) 设计用于试点鱼SSLP的原始剂的跨物种适用性.
- 评估各种鱼物种中简单序列位点中的侧翼序列的保存情况.
主要方法:
- 利用针对Globicephala melas开发的PCR原料,在各种鱼物种中放大同源基位.
- 克隆和测序了来自11种不同的鱼物种 (牙和鱼) 的放大位置.
主要成果:
- 同源的基位被成功地扩大到一个广泛的牙 (Odontoceti) 和 (Mysticeti) 鱼.
- 沿着简单序列线段的序列表现出异常高的保护性,在估计的3500万至4000万年中,平均差异仅为3.2%.
- 这种较低的分歧率表明,有可能重新评估现代鱼物种的进化年龄.
结论:
- 针对一种鱼物种的SSLP设计的PCR原料由于保留了侧翼序列,可以广泛应用于其他鱼物种.
- 这种高度的保护性促进了SSLP DNA指纹的使用,用于在各种类动物中进行基因分析.
- 这些发现突出了鱼物种中非编码DNA的显著低分歧率,促使进一步的进化研究.
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