在杏仁体功能和与恐惧相关的行为中TRPC5的重要作用
Antonio Riccio1, Yan Li, Jisook Moon
1Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children's Hospital Boston, Boston, MA 02115, USA.
Cell
|May 20, 2009
概括
缺乏短暂受体潜能通道5 (TRPC5) 的小鼠表现出降低了先天的恐惧反应. 这表明TRPC5通道对于桃体内由G蛋白结合受体介导的先天性恐惧行为至关重要.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 暂时受体潜能通道5 (TRPC5) 主要存在于大脑中.
- TRPC5通道与TRPC1和TRPC4子单元形成复合体.
- 这些通道具有刺激性,并导出非选择性,由G蛋白结合受体调节.
研究的目的:
- 研究TRPC5在先天性恐惧行为中的作用.
- 为了确定TRPC5通道是否参与与恐惧反应相关的杏仁体内的突触传播.
主要方法:
- 使用了TRPC5淘汰赛 (TRPC5(-/-)) 的小鼠.
- 在对厌恶刺激的反应中评估先天的恐惧水平.
- 检查了杏仁体神经元的突触反应,包括由I组甲基胺和胆固醇基因素2受体介导的反应.
- 测量了突触传输,膜刺激性和杏仁体输入中的长期强化.
主要成果:
- TRPC5 ((-/-) 鼠标表现出明显减少的先天性恐惧.
- 突变小鼠在杏仁体神经元中对I组mGluR和CCK2R激活的突触反应减少.
- 在年轻的TRPC5(-/-) 小鼠中,杏仁体的 afferent输入处的突触强度下降.
- 基线突触传输和兴奋能力在老TRPC5的老TRPC5的老TRPC5的老TRPC5的老TRPC5的老TRPC5的老TRPC5的老TRPC5的老TRPC5.
结论:
- TRPC5在天生的恐惧中起着至关重要的作用.
- 通过G蛋白结合的神经元受体激活TRPC5对于恐惧处理至关重要.
- TRPC5通道对于涉及先天性恐惧反应的特定突触通路至关重要.
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