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相关概念视频

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

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Here, a nanobar-supported lipid bilayer system is developed to provide a synthetic membrane with a defined curvature that enables the characterization of proteins with curvature sensing ability in...
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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

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Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane...
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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method07:26

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Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well...
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Crystallization of Membrane Proteins in Lipidic Mesophases11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

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The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting...
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases22:00

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Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic...
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Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

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The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
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脂质在塑造膜蛋白功能中的新兴作用

Rob Phillips1, Tristan Ursell, Paul Wiggins

  • 1Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA. phillips@pboc.caltech.edu

Nature
|May 22, 2009
PubMed
概括

脂质环境直接影响膜蛋白的结构和功能. 蛋白质诱导的膜形状变化是关键的,能源成本可量化,模型预测得到实验证实.

科学领域:

  • 生物物理学的生物物理.
  • 膜生物学 膜生物学
  • 蛋白质科学 蛋白质科学

背景情况:

  • 膜蛋白与周围的脂质双层有动态相互作用.
  • 脂质-蛋白质相互作用影响蛋白质结构和生物活性.
  • 特定的化学相互作用和物理膜变形有助于这些效应.

研究的目的:

  • 研究脂质环境在膜蛋白功能中的作用.
  • 量化与蛋白质诱导的膜形状变化相关的能量成本.
  • 用实验数据验证理论模型.

主要方法:

  • 利用了膜蛋白及其脂质伙伴的模型系统.
  • 估计了蛋白质诱导的膜扰动的自由能量成本.
  • 在重组系统中测量了通道门的动态.

主要成果:

  • 证实了脂质环境和膜蛋白行为之间的直接联系.
  • 证明蛋白质诱导的膜形状扰动显著影响功能.
  • 实验测量与来自简单生物物理模型的预测保持一致.

结论:

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  • 脂质双层的物理性质,特别是膜形状,是膜蛋白功能的关键决定因素.
  • 量化自由能量计算为脂质-蛋白质相互作用提供了宝贵的见解.
  • 模型系统对于研究复杂的膜蛋白-脂质动态是有效的.