使用蛋白质功能化细胞向原蛋白:多个弱相互作用的强度
Sanne W A Reulen1, Patricia Y W Dankers, Paul H H Bomans
1Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Journal of the American Chemical Society
|May 28, 2009
概括
呈现原结合蛋白 (CNA35) 的小胞表现出增强的稳定性和向原的亲和力. 这突显了多价值相互作用对细胞外矩阵 (ECM) 蛋白向的力量.
科学领域:
- 生物材料科学 生物材料科学
- 生物化学 生物化学
- 纳米技术纳米技术
背景情况:
- 原蛋白是组织重塑和疾病的关键标记物.
- 多价值对自然原相互作用至关重要.
- 菌根是流行的载体,但它们对原蛋白的多价值向尚未得到充分探索.
研究的目的:
- 利用CNA35蛋白质,探索微粒作为向原的多价值支架.
- 为了研究多价值相互作用对菌根-原结合动力学和热力学的影响.
- 评估基于菌根的系统在原向应用中的潜力.
主要方法:
- 使用原生化学结合,制备具有可调节CNA35蛋白质含量的稳定小粒 (0-20个副本/小粒).
- 通过固相和表面等离子体共振试验对CNA35微粒-原结合的热力学和动力学研究.
- 在体外和体外评估细胞稳定性和原亲和力.
主要成果:
- 带有多价值CNA35的菌丝表现出了显著的稳定性,并且在洗时没有与原脱离.
- 由于CNA35.35的多价值显示,观察到对原蛋白亲和力的显著增强.
- 恢复的原亲和力也被观察到与脂质体显示低亲和力CNA35变体在高密度.
结论:
- 多价值相互作用对于有效的基于菌根的原蛋白向至关重要.
- 这项研究表明,结合多种弱相互作用以向多价值细胞外矩阵蛋白的有效性.
- 这种方法对药物输送和向原蛋白的分子成像应用具有前景.
相关概念视频
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagens are the Major Structural Proteins of ECM
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
Connective tissue proper includes loose...
Fibril-associated Collagen
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...

