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基于半孔二氧化纳米粒子的双重药物递送系统,用于对葡萄糖反应的胰岛素和循环AMP的受控释放
Yannan Zhao1, Brian G Trewyn, Igor I Slowing
1Department of Chemistry, U.S. Department of Energy Ames Laboratory, Iowa State University, Ames, Iowa 50011-3111, USA.
Journal of the American Chemical Society
|May 30, 2009
概括
开发了一种使用酸功能化的半孔性纳米颗粒 (BA-MSN) 的新型葡萄糖响应药物递送系统. 这种系统可以控制胰岛素和循环腺单酸盐 (cAMP) 的释放,以应对糖触发.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物输送系统 药物输送系统
背景情况:
- 开发有效的葡萄糖反应系统对于糖尿病管理至关重要.
- 目前的胰岛素输送方法往往缺乏精确的葡萄糖控制.
- 半孔纳米颗粒为药物封装和控制释放提供了多功能平台.
研究的目的:
- 合成和表征一种酸功能化的半孔化纳米粒子 (BA-MSN) 系统,用于双重,对葡萄糖反应的胰岛素和循环腺单酸盐 (cAMP) 的释放.
- 为了研究释放动力学和系统对各种糖类的反应的选择性.
- 评估开发的药物输送系统的体外性能,包括受控释放,细胞毒性和细胞吸收.
主要方法:
- 酸功能化的半孔性二氧化纳米颗粒 (BA-MSN) 的合成.
- 在纳米颗粒表面上固定光素异硫酸标记的葡萄糖酸改性胰岛素 (FITC-G-Ins),并在中介孔内封装cAMP.
- 在体外释放的研究触发的糖 (果糖,葡萄糖) 和在不同的pH条件 (7.4和8.5) 评估.
- 在四个细胞系上使用细胞活力和增殖试验进行细胞毒性评估.
- 使用流细胞计和光共聚焦显微镜进行细胞吸收研究.
主要成果:
- 该BA-MSN系统证明了FITC-G-Ins和cAMP的葡萄糖反应释放.
- 观察到释放选择性,在FITC-G-Ins.中,果糖>葡萄糖>其他糖类.
- 该系统表现出一种独特的双释放机制,其中cAMP释放补偿了FITC-G-Ins在周期中的释放减少.
- 在体外研究证实了pH值7.4和8.5的控制cAMP释放,没有观察到显著的细胞毒性.
- 细胞吸收研究证实了纳米颗粒的有效细胞进入.
结论:
- 成功开发了一种基于BA-MSN的新型葡萄糖反应双释放系统.
- 该系统显示了自我调节的胰岛素输送装置的潜力.
- 进一步的研究可能会导致下一代智能糖尿病管理技术.
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