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在Papaver rhoeas中识别花粉自我不相容性决定因素的识别

Michael J Wheeler¹, Barend H J de Graaf, Natalie Hadjiosif

¹School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

|June 2, 2009

概括

科学家们确定了Papaver rhoeas花粉S (PrpS) 基因,这对自我不相容性至关重要. 该基因编码了一种跨膜蛋白,该蛋白与杆S决定体 (PrsS) 相互作用,抑制不兼容的花粉,并防止植物的近亲繁殖.

科学领域:

植物生殖生物学植物生殖生物学
分子遗传学分子遗传学
进化生物学是进化的生物学.

背景情况:

较高的植物依赖于花粉-的相互作用来生产种子.
自我不相容性 (SI) 是一个关键的遗传机制,防止内生繁殖,由S位点控制.
在Papaver rhoeas中,状S-locus蛋白触发一种依赖的信号级联,抑制不兼容的花粉.

研究的目的:

为了识别和描述Papaver rhoeas.中的花粉S-locus决定性.
阐明S特异性花粉识别和抑制的分子机制.
了解自我不相容系统的进化起源.

主要方法:

克隆了由花粉表达基因PrpS (Papaver rhoeas花粉S) 的三个等位基因.
对PrpS的序列分析及其与pistil S基因 (PrsS) 的联系.
生物化学测试以确定PrpS蛋白质的特性和相互作用,包括使用反感性寡核酸.

主要成果:

鉴定出PrpS基因是花粉S位点的决定因素.
PrpS编码了一种新型,大约20kDa的与血膜相关联的跨膜蛋白.
一个PrpS的细胞外循环与状S决定物 (PrsS) 相互作用,调解S特异性花粉抑制.

结论:

在Papaver rhoeas.as.中,PrpS对于S特异性抑制不兼容花粉至关重要.
鉴定PrpS为自我不相容的分子基础提供了重要的洞察力.
PrpS和PrsS是古老的,共同进化的基因,有助于理解细胞对细胞识别系统.

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