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相关概念视频

Redox Reactions01:27

Redox Reactions

Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
Redox Reactions01:24

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
Redox Equilibria: Overview01:23

Redox Equilibria: Overview

A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

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相关实验视频

Updated: Jun 22, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

在核糖核酸还原酶的氧化还原相关结构变化.

A R Offenbacher1, I R Vassiliev, M R Seyedsayamdost

  • 1Department of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.

Journal of the American Chemical Society
|June 4, 2009
PubMed
概括
此摘要是机器生成的。

рибо核酸减少酶 (RNR) 使用铁基 (Y122*) 启动催化. 这项研究揭示了附近的氨基酸键的氧化还原连接结构变化,这是由基的静电变化驱动的.

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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

相关实验视频

Last Updated: Jun 22, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 频谱学是一种光谱学.

背景情况:

  • рибо核酸减少酶 (RNR) 对于DNA合成至关重要,催化了脱氧核酸的产生.
  • 像大肠杆菌β2亚单元一样,I类RNR具有铁中心和用于催化的关键铁自由基 (Y122*).
  • 在Y122*中,氧依赖的形状变化可能调节质子合电子转移.

研究的目的:

  • 调查与大肠杆菌β2.2中的Y122*基相关的氧化还原相关结构变化.
  • 了解铁基在启动催化反应中的作用.
  • 探索质子合电子转移调节的机制.

主要方法:

  • 福利埃变换红外光谱法 (FT-IR) 用于检测反应诱导的光谱变化.
  • 同位素标记用 (2) H ((4) 氨酸和 (15) N 氨酸有助于光谱分配.
  • 差异光谱是在Y122*通过基尿素减少时获得的.

主要成果:

  • FT-IR分析确定了与Y122 (1514 cm(-1)) 和Y122* (1498 cm(-1)) 相关的特定振动频段.
  • 反应诱导的光谱显示了胺I波段的变化 (1661和1652 cm(-1)),表明结构变化.
  • 这些胺带转移反映了在模型五中观察到的变化,表明了序列介导效应.

结论:

  • Y122*的减少与附近的胺键的结构性扰动相结合.
  • 这些结构变化受到围绕着铁基的氨基酸序列的影响.
  • 一种拟议的机制涉及铁基的芳香环内与氧化还原相关的静电变化,导致胺键扰动.