一个管状生物容器:一种分子工程 chaperonin 的金属离子诱导的 1D 组件
Shuvendu Biswas1, Kazushi Kinbara, Nobuhiro Oya
1Department of Chemistry and Biotechnology, School of Engineering and Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Journal of the American Chemical Society
|June 4, 2009
概括
修改后的GroEL蛋白与光色单元自组装成长,空洞的纤维. 这些蛋白质纤维可以被分解并与变质蛋白质结合,形成功能性蛋白质纳米结构.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
背景情况:
- I 组护卫蛋白 GroEL 在蛋白质折叠中起着至关重要的作用.
- 光色分子,如螺旋 (SP) 和墨氨酸 (MC),在暴露于光线时可以改变它们的特性.
- 对蛋白质的特定修改允许创建新的功能材料.
研究的目的:
- 为了设计一个修改的GroEL蛋白,GroEL ((SP/MC),与集成的光色单元.
- 为了研究GroEL的自组装行为 (SP/MC) 作为对双价金属离子的反应.
- 探索GroEL (SP/MC) 组件在绑定和封装变质蛋白质方面的潜力.
主要方法:
- 通过基因和化学修饰,将大约28个螺旋 (SP) 和梅洛 (MC) 单元连接到GroEL蛋白洞口.
- 通过将双价金属离子 (例如,Mg2+) 添加到缓冲溶液中,诱导了GroEL (SP/MC) 的一维 (1D) 组装.
- 使用EDTA实现了组装纤维的拆卸.
- 评估了GroEL ((SP/MC) 对变质蛋白的结合亲和力,并形成了含有客体的纤维.
主要成果:
- GroEL ((SP/MC) 在特定地点成功地整合了光色单元.
- 二元金属离子诱导了GroEL ((SP/MC) 的长圆柱状空心纤维的形成,具有高面比 (高达2.5微米).
- 治疗EDTA有效地将纤维分解成较小的寡合体和单体.
- GroEL ((SP/MC) 保持了与非化的蛋白质结合的能力,形成蛋白质载荷的纤维.
结论:
- 在GroEL中对光色单元的特定场所的整合使得自控自组装成为有序的纳米结构.
- 这些GroEL ((SP/MC) 纤维具有可调节的组装和拆卸特性.
- 由此产生的基于蛋白质的纤维显示出在蛋白质结合和输送系统中的应用潜力.
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