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相关概念视频

Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Introduction To Enzymes01:22

Introduction To Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...

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相关实验视频

Updated: May 13, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

在多基化物多样化中的变态酶组合.

Liangcai Gu1, Bo Wang, Amol Kulkarni

  • 1Life Sciences Institute, Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.

Nature
|June 5, 2009
PubMed
概括
此摘要是机器生成的。

在Lyngbya majuscula中的酶进化了并行路径,以创建独特的化学结构,如环和乙烯化物. 这项研究揭示了酶修饰如何推动天然产品的多样性.

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相关实验视频

Last Updated: May 13, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

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科学领域:

  • 生物化学 生物化学
  • 自然产品生物合成 自然产品生物合成
  • 酶学 是一种酶学.
  • 化学生物学 化学生物学

背景情况:

  • 自然产品的多样性源于二次新陈代谢中的生物合成途径的演变.
  • 推动代谢多样化的酶的共同进化在生物化学层面上仍然不太了解.
  • 林格比亚巨型通过复杂的酶过程产生复杂的二次代谢产物.

研究的目的:

  • 通过生物化学方法研究Lyngbya majuscula中环烯和乙烯化物形成的机制.
  • 了解参与多基酸β分支和化过程中的酶的共同进化.
  • 阐明导致二次代谢物中功能组多样性的并行进化事件.

主要方法:

  • 关键酶的生物化学评估,包括酶,脱水酶 (ECH(1)),脱碳酶 (ECH(2) 和乙烯基还原酶域.
  • 来自Lyngbya majuscula. 的并行生物合成途径 (库拉辛A和牙买卡米德途径) 的分析.
  • 在形成β分支环和乙烯基化物部分的酶活动的表征.

主要成果:

  • 一个基酶在多基酸β分支路径中引入了马化步骤.
  • ECH(2) 酶的不同活动导致了α,β或β,gamma enoyl thioester的形成.
  • 一个乙烯基还原酶域催化了前所未有的环化反应,形成了一个环环.

结论:

  • 化,多基化β分支和酶机制多样化的结合产生了环和乙烯基化物部分.
  • 多酶系统的并行进化推动了自然产品中的功能组多样性.
  • 这项研究提供了对代谢多样化酶的共同进化的生物化学见解.