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在粉样蛋白的交叉β槽中模拟分子阵列

W Seth Childers¹, Anil K Mehta, Kun Lu

¹Center for Fundamental and Applied Molecular Evolution, and Department of Chemistry and Biology, Emory University, Atlanta, Georgia 30322, USA.

Journal of the American Chemical Society

|July 3, 2009

概括

刚果红 (CR) 与粉样结构的结合被使用交叉β纳米管研究. 这项研究澄清了CRCR.

科学领域:

生物化学生物化学
结构生物学结构生物学
生物物理学的生物物理.

背景情况:

粉样纤维具有共同的交叉β结构,并与刚果红色 (CR) 结合,这是一种对诊断至关重要的染料.
由于纤维的不溶性和异质性,CR与粉样结构的精确结合相互作用仍然不太清楚.

研究的目的:

为了阐明刚果红色和粉样蛋白结构之间的特定结合相互作用.
为了描述CR与粉样蛋白结合时的方向和聚合状态.

主要方法:

使用交叉β纳米管来限制潜在的CR结合点.
采用了刚果红色结合测定,电子衍射和线性二极化.
进行了半实证量子计算以建模CR聚合.

主要成果:

与交叉β纳米管结合的CR表现出特有的果绿色干扰颜色和1.9 +/- 0.5微米的K(d).
电子衍射和线性二极化确定了与粉体长轴并行的CR方向和层状槽.
量子计算支持J-和H-CR聚合物的形成.

结论:

粉状结构将CR组织成精确的J和H聚合物网络.
这种分子组织解释了刚果红色在粉样蛋白检测中的诊断效用.

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