Jmjd6催化了U2AF65的lysyl-hydroxylation,这种蛋白与RNA剪接相关
Celia J Webby1, Alexander Wolf, Natalia Gromak
1Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK.
概括
朱蒙吉域-6蛋白 (Jmjd6) 氧化了U2小核核核糖核蛋白辅助因子65千多子单元 (U2AF65),这是一个拼接因子. 这种由Jmjd6的基化特别调节了替代RNA剪接.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 翻译后修改 翻译后修改
背景情况:
- 甲基动物的低氧反应由依赖氧气的低氧诱导因子 (HIF) 的翻译后化法调节.
- 这种调控影响HIF活性和寿命,促使对参与基因表达控制的其他基酶进行研究.
研究的目的:
- 研究Jumonji域-6蛋白 (Jmjd6) 在翻译后修饰和基因表达调节中的作用.
- 为了确定Jmjd6是否催化了HIF以外的其他蛋白质的氧化.
主要方法:
- 研究了U2小核核核糖核蛋白辅助因子65千多子单位 (U2AF65) 的翻译后修改.
- 使用生物化学测试来确定负责U2AF65.65的lysyl-5-hydroxylation的酶.
- 评估Jmjd6活动对内源和记者基因的替代RNA剪接的影响.
主要成果:
- 确定了Jmjd6作为Fe(II) 和2-氧格酸盐依赖的二氧化酶,催化了U2AF65.65的lysyl-5-hydroxylation.
- 证明Jmjd6是一种核蛋白,在脊椎动物发育中起作用,类似于其在HIF氧化中参与的同类蛋白.
- 表明Jmjd6改变了基因子集的替代RNA剪接.
结论:
- Jmjd6在通过拼接因子U2AF65.6的翻译后基化来调节替代RNA拼接方面发挥着特定的作用.
- 这一发现将Jmjd6已知的功能扩展到HIF调节之外,并强调其在基因表达控制中的重要性.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


