相关实验视频
Updated: Jun 21, 2026

08:01
The Soft Agar Colony Formation Assay
Published on: October 27, 2014
通过LEF1和HOXB9传递WNT/TCF信号,调解肺腺癌转移的发生
Don X Nguyen1, Anne C Chiang, Xiang H-F Zhang
1Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Cell
|July 7, 2009
概括
WNT/TCF通路驱动肺腺癌快速转移到大脑和骨. 针对这种途径,特别是HOXB9和LEF1,可以抑制癌症的传播.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症转移 癌症转移
背景情况:
- 肺腺癌可以迅速转移到多个器官.
- 驱动这种快速转移能力的潜在机制在很大程度上是未知的.
- 了解这些机制对于开发有效的治疗策略至关重要.
研究的目的:
- 确定负责快速肺腺癌转移的分子机制.
- 研究WNT/TCF途径在大脑和骨转移中的作用.
- 为了确定参与介导转移的特定WNT/TCF基因.
主要方法:
- 在初级肺腺癌中分析基因表达特征.
- 从细胞系中分离和表征转移性亚种群.
- 使用小鼠模型进行体内研究,以评估WNT/TCF通路调节对转移的影响.
- 识别和验证WNT/TCF目标基因.
主要成果:
- 标准WNT/TCF通路的激活是肺腺癌转移到大脑和骨的关键决定因素.
- 在初级瘤中,WNT/TCF通路激活信号与多器官复发相关.
- 在转移性亚种群中观察到过度活跃的WNT/TCF通路.
- 降低TCF活性降低了小鼠形成脑和骨转移的能力,独立于原发性瘤生长.
- 确定HOXB9和LEF1是关键的WNT/TCF目标基因,它们可以调解入侵和殖民.
结论:
- WNT/TCF信号通路在使肺腺癌细胞转移到大脑和骨方面发挥着至关重要的作用.
- 特定的WNT/TCF点基因HOXB9和LEF1是这种转移过程的重要媒介.
- 准WNT/TCF途径及其下游效应器是一个潜在的治疗策略,可以抑制肺腺癌转移.
相关概念视频
Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Metastasis
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
