光系II中的质子合电子转移:对氧化还原活性氨酸的质子库存
David L Jenson1, Bridgette A Barry
1School of Chemistry and Biochemistry and the Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Journal of the American Chemical Society
|July 10, 2009
概括
摄影系统II 摄影系统II
科学领域:
- 生物化学 生物化学
- 光合作用研究研究光合作用.
- 蛋白质生物物理学 蛋白质生物物理学
背景情况:
- 光系统II (PSII) 对于光合作用至关重要,涉及水的氧化和塑基的减少.
- D1和D2聚构成了PSII的核心,氧进化复合体 (OEC) 负责水的氧化.
- 氨酸Y(D) 参与OEC稳定,并与histidine 189一起表现出质子合电子转移 (PCET).
研究的目的:
- 在高pH下研究光系统II中Y(D) 质子合电子转移 (PCET) 的机制.
- 阐明Y(D) 氧化还原反应中涉及的质子转移途径.
主要方法:
- 利用质子库存技术在高pH下分析Y(D) PCET.
- 在之前的工作中应用了同位素标记和位点定向突变发生,以分配Y (D) 并研究其相互作用.
主要成果:
- 质子库存数据显示过度曲线,表明多个质子捐赠途径到Y
- 至少有一条途径涉及多个质子的转移.
- 在高pH下,PCET机制比以前理解的要复杂得多.
结论:
- 这些发现揭示了光系统II的Y(D) PCET中复杂的质子转移动态.
- 多个质子通路,包括多个质子转移,对于在高pH下Y(D) 功能至关重要.
- 这项研究推动了我们对光合作用水氧化背后的分子机制的理解.
相关概念视频
Photosystem II
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Photosystem I
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
The Photochemical Reaction Center
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
The Z-Scheme of Electron Transport in Photosynthesis
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
Photosystems
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Electron Transport Chain: Complex III and IV
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...

