固定网林-1的引力足以重新定位轴突
Simon W Moore1, Nicolas Biais, Michael P Sheetz
1Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA.
Science (New York, N.Y.)
|July 11, 2009
概括
分泌的指导线索,如netrin-1,可以为发展中轴突提供引力. 固定的netrin-1使生长能够产生显著的力量,在胚胎发育过程中重定向轴突轨迹.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 轴指导在胚胎发育期间的神经电路形成至关重要.
- 分泌的细胞外基质线索,如netrin-1,引导成长的轴突到他们的目标.
- 分泌线索在为生长提供物理引力的作用在很大程度上仍未被探索.
研究的目的:
- 调查是否分泌的指导线索,特别是netrin-1,可以为生长提供物理引力.
- 量化增长在固定网-1.0上产生的力量.
主要方法:
- 利用先进的显微镜和力测量技术来分析生长的行为.
- 面对前进的脊柱 commissural 神经元生长与固定网林-1 基质.
- 测量了成长对网林-1基板施加的引力.
主要成果:
- 脊柱 commissural 神经元生长产生了415 piconewtons的基线力.
- 在不动化的netrin-1的存在下,生长产生了显著更高的引力,超过63 piconewtons.
- 这些增加的力足以重定向轴突的轨迹.
结论:
- 分泌的指导线索,当在细胞外基质中固定时,可以充当粘合基质.
- 网林-1提供了大量的物理引力,积极影响了轴突路径.
- 这一发现揭示了在发育过程中支轴突指导的新型机械机制.
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