病毒囊体DNA合体结合为多价值细胞向载体
Gary J Tong1, Sonny C Hsiao, Zachary M Carrico
1Department of Chemistry, University of California, Berkeley, and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, California 94720-1460, USA.
Journal of the American Chemical Society
|July 17, 2009
概括
研究人员开发了一种新的氧化合方法,将核酸体连接到病毒体,以向药物输送. 这种aptamer功能化囊系统显示出向特定细胞输送酸性无效药物的承诺,以释放 lysosomal.
科学领域:
- 生物结合化学 生物结合化学
- 纳米医学是一种纳米医学.
- 分子准是指分子准.
背景情况:
- 核酸体是用于药物输送应用的多功能向部分.
- 病毒囊体作为治疗有效载荷的强大的载体.
- 需要有效的方法来将体与病毒囊表面结合起来.
研究的目的:
- 开发一种高效的氧化合策略,用于将体与病毒体结合起来.
- 为了表征aptamer功能化的病毒体的稳定性和功能性.
- 为了评估aptamer标记的囊剂在向药物输送方面的潜力.
主要方法:
- 定期介导的氧化合的烯二胺修饰的寡核酸与林功能化的病毒囊.
- 鉴定DNA链附着密度和基因配对能力的保留.
- 使用Jurkat T细胞和氨酸激酶受体向性受体的细胞结合试验.
- 协焦显微镜用于同位化研究和细胞吸收和贩运的评估.
主要成果:
- 通过氧化合方法,成功地将每种病毒囊连接到每种病毒囊的多达60个DNA合酶链.
- 证明了合后的阿巴胺基配对能力和囊蛋白稳定性的保留.
- 已证实,阿普他默功能化体与细胞 (Jurkat T 细胞) 的特定结合.
- 观察到阿普坦-囊复合体的内细胞和溶酶体贩运.
结论:
- 开发的氧化合策略提供了一个有效的手段来创建aptamer功能化的病毒囊.
- 标有Aptamer标签的病毒囊可以专门向细胞并经历内细胞分裂.
- 这些胺体-体结构具有针对性药物递送的潜力,特别是在溶酶体中释放的酸性无效前药物.
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