基因聚合酶V的活性形式是 UmuD'(2)C-RecA-ATP
Qingfei Jiang1, Kiyonobu Karata, Roger Woodgate
1Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California 90089-2910, USA.
Nature
|July 17, 2009
概括
RecA*将ReCA-ATP转移到DNA聚合酶V (pol V) 中,以创建一个活跃的突变体,使转移DNA合成成为可能. 然后,这种突变酶独立于ReCA*运作,其活动受到ReCA-ATP交换的调节.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 大肠杆菌中的SOS突变发生涉及DNA聚合酶V (pol V) 和RecA核蛋白丝 (RecA*).
- 活性聚合物V的精确分子组成和ReCa*在突变发生过程中的确切功能仍然不清楚.
研究的目的:
- 为了阐明pol V.的活性变异原体形式的分子组成.
- 确定ReCA*在激活SOS突变发生的PolV中的特定作用.
主要方法:
- 聚合物V Mut复合物的生物化学复合.
- 对RECA-ATP转移进行静脉测量分析.
- 对pol V Mut活性和失活性的动力学研究.
主要成果:
- RecA*将单个ReCA-ATP分子转移到自由聚V (UmuD'(2) C中,形成具有UmuD'(2) C-ReCA-ATP组成的活性突变体 (pol V Mut).
- 聚乙基突变催化转化合成 (TLS) 独立于ReCA*.
- 在DNA解离时,Pol V Mut会迅速失活,在DNA合成缺失时,失活速度较慢,并保留ReCA-ATP.
结论:
- 在SOS突变发生过程中,ReCA*的主要作用是将ReCA-ATP转移到pol V.
- 这种转移产生了转化合成所必需的活性突变体综合体.
- 波尔V Mut活动是由ReCA-ATP的交换调节的,突出了控制突变发生的新机制.
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