相关实验视频
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High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
一个启动器结合转录启动因子和螺旋环螺旋激活器USF的合作相互作用
A L Roy1, M Meisterernst, P Pognonec
1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Nature
|November 21, 1991
概括
研究人员发现了一种新的转录因子,TFII-I,它与发起体 (Inr) 元素结合. 这一因素在基底转录中发挥作用,并与USF相互作用,在基因调节中提出了新的通信途径.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 生物化学 生物化学
背景情况:
- 通过RNA聚合酶II的转录启动涉及常见的因素和最小的促进元件,如TATA盒和启动器 (Inr).
- 绑定TFIID到TATA元件有助于预启动复杂组装.
- 在Inr元素中运行的因素仍然未被定义.
研究的目的:
- 识别和描述涉及到在Inr元素的转录启动中的新型因素.
- 研究这些因素在基底转录中的作用及其与其他已知的转录因子的关系.
主要方法:
- 发现了一种新型的转录因子TFII-I,该因子特别与Inr元素结合.
- 试验证明了TFII-I对从腺病毒主要晚期促进体的基底转录的支持.
- 免疫学和结合性研究,以探索TFII-I,USF和它们各自的结合部位之间的关系 (Inr和E框).
主要成果:
- 一个新的因素,TFII-I,被确定并被证明是专门与Inr元素结合的.
- TFII-I支持基底转录,并且在免疫学上与螺旋环螺旋激活器USF有关.
- TFII-I和USF在Inr和E盒子站点展示了合作绑定,表明了交叉相互作用.
结论:
- TFII-I代表了一类新的转录启动因子.
- TFII-I在多个促进元的相互作用表明了调节因素和一般转录机制之间的新型通信机制.
- TFII-I和USF的合作绑定凸显了促进地区复杂的监管相互作用.
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