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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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相关实验视频

Updated: Jun 21, 2026

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

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用于标记和释放的醇选择性化探针.

Vu Hong1, Alexander A Kislukhin, M G Finn

  • 1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Journal of the American Chemical Society
|July 23, 2009
PubMed
概括
此摘要是机器生成的。

研究人员开发了新的7-oxanorbornadiene (OND) 探针,用于在水溶液中对醇进行选择性和化标记. 这些探头具有很高的反应性,并能够在和蛋白质中灵敏地检测.

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TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
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科学领域:

  • 化学生物学 化学生物学
  • 有机化学 有机化学
  • 生物化学 生物化学

背景情况:

  • 醇化对于蛋白质标记至关重要.
  • 现有的方法可能缺乏选择性或需要特定条件.
  • 需要开发高效和多功能的醇反应探针.

研究的目的:

  • 引入一类新的基于7-oxanorbornadiene (OND) 的探针.
  • 为了在中性pH的水溶液中实现选择性,化标签的硫醇.
  • 探索裂变机制和由此产生的的多功能性.

主要方法:

  • 合成7-oxanorbornadiene (OND) 衍生物与丹西尔光体.
  • 研究硫醇添加动力学和光增强.
  • 分析反复-迪尔斯-阿尔德碎片化和苏奇尼米德的形成.
  • 在和蛋白质中对硫醇进行化学选择性标记.

主要成果:

  • OND探针具有高反应性 (40-200 M(-1) s(-1)) 和对醇的选择性.
  • 光强度在添加硫醇时增加了多达180倍.
  • 吸管允许通过逆-迪尔斯-阿尔德或苏尼米德的形成进行裂变,从而使酒精释放.
  • 蛋白质和的成功标记与OND电友.

结论:

  • 该OND框架为开发反应性和原性醇探针提供了一个多功能平台.
  • 这些探针能够在生物系统中灵敏地检测和标记醇.
  • OND链接器的模块化性质允许进一步的功能化和其他分子的附着.