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颜色学分离G-四重复探头用于核酸传感:通过探头重塑来提高重组DNAzyme的催化效率

Shizuka Nakayama1, Herman O Sintim

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.

Journal of the American Chemical Society
|July 23, 2009
PubMed
概括

分裂G丰富的DNA探针形成活性DNA酶,用于简单的生物分析检测. 优化探针结构和反应条件可以提高酶的效率和稳定性,从而改善生物测试.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...