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相关概念视频

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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相关实验视频

Updated: Jun 21, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

通过纳米粒子合DNA模板反应的多重复单核酸多态型类型化.

Xuejia Xue1, Wei Xu, Feng Wang

  • 1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.

Journal of the American Chemical Society
|August 1, 2009
PubMed
概括

这项研究引入了一种新的基于芯片的方法,用于使用纳米粒子合DNA结合的单核酸多态 (SNP) 类型. 这种方法提供了快速,灵敏和经济高效的多重SNP检测,无需复杂的程序.

科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断
  • 纳米技术纳米技术

背景情况:

  • 单核酸多态 (SNP) 是重要的遗传标记.
  • 现有的SNP打字方法通常需要昂贵的设备和复杂的协议.
  • 需要使用高效,灵敏和具有成本效益的SNP检测技术.

研究的目的:

  • 为SNP类型开发一种基于芯片的新型检测方法.
  • 利用纳米粒子合的DNA模板绑定反应进行增强检测.
  • 为多重SNP基因定型提供快速,灵敏和成本有效的替代方案.

主要方法:

  • 一个基于芯片的平台被设计用于SNP检测.
  • 纳米粒子合DNA探针被使用.
  • 用DNA模板绑定反应来识别SNP.
  • 该方法被优化,以避免昂贵的仪器仪表和严格的洗步骤.

主要成果:

  • 这种新的方法证明了快速多重SNP检测能力.
  • 在识别单基不匹配时,实现了超高灵敏度.
  • 该方法成功地确定了精确的单基不匹配位置.

更多相关视频

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

相关实验视频

Last Updated: Jun 21, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

  • 程序被简化,消除了复杂的严格洗的需要.

    • 结论:

    • 开发的基于芯片的结合试验为高通量多重SNP基因定型提供了一种时间效率高的方法.
    • 这种方法提供了一个具有成本效益和高度敏感的替代传统SNP打字技术.
    • 这项技术有可能显著推进遗传分析和诊断.