第I类主要基因相容性复合体被周期性触发器加载
Boris Rodenko1, Mireille Toebes, Patrick H N Celie
1Division of Cell Biology II, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Journal of the American Chemical Society
|August 7, 2009
概括
研究人员开发了一种新的化学触发方法来创建定义的-MHC,克服了基于UV的方法的局限性,用于T细胞治疗和高通量查等应用.
科学领域:
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 第I类主要基因相容性复合体 (MHCs) 对于向细胞毒性T细胞呈现来说至关重要.
- 目前用于生成重组MHC的方法需要在体外重新折叠过多的,限制高吞吐量生产.
- 光联体提供有条件的MHC联体交换,但受到紫外线透和细胞培养中的热量问题的阻碍.
研究的目的:
- 开发一种新的化学触发诱导方法来产生定义的-MHCs.
- 克服基于紫外线的配体交换对MHC复合体生产的局限性.
- 为了实现T细胞治疗和高通量查中的应用.
主要方法:
- 设计和合成一种用于MHC分子的新型化学敏感联结体.
- 对MHC-连接体复合物的晶体分析,以确认结合部位占用和连接体反应性.
- 化学触发方法的验证,用于生产用于T细胞检测的-MHC.
主要成果:
- 一个晶体结构证实了化学敏感联体与MHC结合在一个反应性构造.
- 化学触发方法成功生成了定义的MHCs.
- 产生的MHC被验证用于T细胞检测.
结论:
- 化学触发诱导的配体交换为生成定义的MHCs提供了一种多功能和适用的方法.
- 这项技术促进了MHC在细胞培养中进行载入,以便在细胞治疗中进行T细胞扩张和净化.
- 该方法支持开发小型化系统,用于MHC配体的高通量选.
相关概念视频
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
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Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
Cell-mediated Immune Responses
Overview
Antigen Presenting Cells
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...


