小分子链接DNA的终端保护,用于通过选择性碳纳米管组件通过敏感的电化学检测蛋白质结合

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Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
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Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

Zhan Wu1, Zhen Zhen, Jian-Hui Jiang

  • 1State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Journal of the American Chemical Society
|August 7, 2009
PubMed
概括

一种新的测定方法可以保护小分子结合的DNA在与其蛋白质标结合时免受降解. 这使得能够灵敏地检测小分子-蛋白相互作用,如叶酸和叶酸受体,使用DNA序列放大.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...