种群水平的转录周期来自单细胞转录的随机时间
Tatjana Degenhardt1, Katja N Rybakova, Aleksandra Tomaszewska
1Department of Biosciences, University of Kuopio, 70211 Kuopio, Finland.
Cell
|August 12, 2009
概括
这项研究揭示了基因转录中的60分钟周期,由基因组修饰和RNA聚合酶II活性等分子事件驱动. 这种内在的周期性解释了种群水平的基因表达模式.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物物理学的生物物理.
背景情况:
- 细胞转录是一个复杂的,依赖蛋白质的过程.
- 了解单个分子水平的基因表达动态至关重要.
研究的目的:
- 模拟和理解周期性基因转录背后的分子机制.
- 研究人类细胞中与转录相关的过程的周期性.
主要方法:
- 为单基因转录开发了详细的随机模型.
- 测量了pyruvate脱酶激酶4基因的循环表达.
- 结合了扩散和蛋白质复合体动态的现实动力学.
主要成果:
- 观察到大约60分钟的周期性基因组修饰,调节蛋白质存在,RNA聚合酶II活性,染色蛋白循环形成和mRNA积累.
- 该模型准确地预测了导致人口水平转录循环的单基因活动时间.
- 确定了转录启动的顺序性和不可逆转性质是关键.
结论:
- 转录过程的内在周期性是人口水平基因表达循环的主要驱动因素.
- 这项研究为基因表达节奏提供了分子层面的理解.
相关概念视频
Structure of a Gene
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...


