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成年小鼠由诱导的多能干细胞产生

Michael J Boland¹, Jennifer L Hazen, Kristopher L Nazor

¹Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

|August 13, 2009

概括

诱导多能干细胞 (iPSC) 现在可以产生完全肥沃的成年小鼠,显示出完全的多能性. 这一突破证实了iPSC在再生医学和疾病建模方面的有效性,没有道德问题.

科学领域:

干细胞生物学干细胞生物学
生殖生物学生殖生物学
发展生物学发展生物学

背景情况:

转录因子的短暂过度表达可以产生类似胚胎干细胞 (ESC) 的诱导多能干细胞 (iPSC).
在不使用胚胎组织的情况下,iPSC为患者特定的细胞疗法和疾病建模提供了潜力.
目前的iPSC在四平状体补充试验中没有持续生成成年小鼠,质疑它们的全部发育潜力.

研究的目的:

确定直接重新编程是否可以实现社会经济委员会充分的多元性.
从重新编程的细胞完全产生完全发育的,肥沃的成年小鼠.
建立一个资源来比较多能状态和验证iPSC应用程序.

主要方法:

诱导性基因重编程小鼠胚胎纤维细胞以产生iPSCs.
四体补充测试以评估生成的iPSCs的发展潜力.
完全从iPSCs获得的成年小鼠的生成和分析.

主要成果:

成功生成了完全来自iPSCs的肥沃成年小鼠.
证明分化细胞可以被重新编程到相当于ESC的多能性水平.
确立了重编程可以在没有 ooplasmic 因素的情况下发生.

结论:

直接重编程可以恢复足以产生整个生物体的多能性.
完全多能性的iPSC是可以实现的,验证了它们在再生医学中的潜力.
通过比较不同的iPSC线条,可以识别多能性状态的标记物,并评估治疗应用的基因组稳定性.

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