在非A,非B型肝炎中检测型肝炎病毒序列
A J Weiner1, G Kuo, D W Bradley
1Chiron Corporation, Emeryville, California 94608.
Lancet (London, England)
|January 6, 1990
概括
在缺乏抗C100-3抗体的患者中检测到C型肝炎病毒 (HCV) RNA,这表明HCV感染的低估. 这表明目前的抗体测试可能会错过许多感染病例.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 肝炎C病毒 (HCV) 是输血后非A,非B型肝炎 (NANBH) 的重要原因.
- 对HCV感染的诊断一直依赖于检测抗体,如抗C100-3和病毒RNA.
研究的目的:
- 调查HCV在输血后NANBH中的作用.
- 评估抗C100-3抗体检测在识别HCV感染和传染性方面的敏感性.
主要方法:
- 从NANBH患者和实验感染黑猩猩的临床样本的分析.
- 使用cDNA/聚合酶链反应检测HCVRNA.
- 使用放射性免疫检测检测C100-3的抗体.
主要成果:
- 在患者中检测到HCVRNA,对抗C100-3抗体呈阴性.
- 传染性HCVRNA在血中被发现来自血清阴性个体和黑猩猩.
- 在10名抗C100-3阳性慢性NANBH患者中,有7名具有病毒序列.
结论:
- 反C100-3抗体是HCV感染性的标志物.
- 当仅依赖于抗C100-3抗体频率时,可能会低估HCV感染.
- 检测HCVRNA对于准确诊断至关重要,特别是在血清阴性病例中.
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