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相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Facilitated Diffusion01:16

Facilitated Diffusion

The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...

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相关实验视频

Updated: Jun 21, 2026

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
11:58

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Published on: February 29, 2012

膜蛋白的侧向扩散 膜蛋白的侧向扩散

Sivaramakrishnan Ramadurai1, Andrea Holt, Victor Krasnikov

  • 1Department of Biochemistry, Groningen Biomolecular science and Biotechnology Institute & Zernike Institute of Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Journal of the American Chemical Society
|August 14, 2009
PubMed
概括

测量了巨型单囊 (GUVs) 中的整膜蛋白的移动性. 随着度的增加,蛋白质扩散会减缓,在低密度下遵循萨夫曼-德尔布鲁克模型.

更多相关视频

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

相关实验视频

Last Updated: Jun 21, 2026

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
11:58

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Published on: February 29, 2012

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

科学领域:

  • 生物物理学的生物物理.
  • 膜生物学 膜生物学
  • 蛋白质动力学 蛋白质动力学

背景情况:

  • 整体膜蛋白对于细胞功能至关重要.
  • 了解它们的横向运动是理解膜组织和功能的关键.
  • 像萨夫曼-德尔布鲁克 (Saffman-Delbruck) 等先前的模型为脂质双层中蛋白质扩散提供了一个框架.

研究的目的:

  • 量化各种一体膜蛋白在巨型单囊泡 (GUVs) 中的横向移动性.
  • 研究蛋白质度和大小对扩散系数的影响.
  • 评估萨夫曼-德尔布鲁克模型在不同条件下的适用性.

主要方法:

  • 使用光相关谱法 (FCS) 来测量横向扩散.
  • 综合膜蛋白 (受体,通道,传输体) 和一个α-螺旋被光标记.
  • 蛋白质在不同的蛋白质与脂质比率下被重新构成GUV.

主要成果:

  • 在低蛋白质密度 (10-100蛋白/μm2) 的情况下,扩散系数对蛋白质半径的依赖性很弱,与萨夫曼-德尔布鲁克模型保持一致.
  • 在高蛋白密度 (高达3000蛋白/μm2) 时,随着蛋白质度的增加,横向扩散线性减少.
  • 研究结果表明,蛋白质拥挤对移动性有重大影响.

结论:

  • 蛋白质与蛋白质的相互作用和膜拥挤显著影响横向运动.
  • 扩散测量可以用来推断蛋白质几何和寡合化状态.
  • 这项研究提供了与拥挤的生物膜相关的蛋白质动态的见解.