在CD4+调节性T细胞中,Eos调解了Foxp3依赖基因沉默
Fan Pan1, Hong Yu, Eric V Dang
1Immunology and Hematopoiesis Division, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
Science (New York, N.Y.)
|August 22, 2009
概括
调节性T细胞 (Tregs) 使用转录因子Eos使基因沉默,保持免疫恒常. Eos对于Treg功能至关重要,并防止过度的免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- CD4+调节性T细胞 (Tregs) 对于维持免疫平衡和自我耐受性至关重要.
- 转录因子Foxp3控制Treg功能,但对基因抑制机制的了解很少.
- 了解Treg基因抑制是控制异常免疫反应的关键.
研究的目的:
- 在Tregs中识别Foxp3-依赖基因抑制的新型媒介.
- 阐明Treg介导免疫抑制的基础分子机制.
- 调查Treg编程和功能中确定的因素的作用.
主要方法:
- 同免疫沉测试检测蛋白质与蛋白质相互作用.
- 染色体免疫沉降测序 (ChIP-seq) 用于识别目标基因.
- 在Tregs中进行基因沉默研究,以评估功能后果.
- 分析与基因抑制相关的染色质修饰.
主要成果:
- 伊卡罗斯家族的指转录因子Eos被确定为Tregs.中的关键抑制剂.
- 埃奥斯直接与Foxp3相互作用,诱导基因沉默.
- 通过eos介导的沉默涉及到特定的染色质修饰.
- 在Tregs中失去Eos会损害它们的抑制功能,并赋予部分效应特征.
结论:
- 在Tregs.中,Eos是Foxp3-依赖基因沉默的关键调解者.
- 在Treg编程和维持免疫平衡中,Eos起着至关重要的作用.
- 针对Eos可能为免疫相关疾病提供治疗策略.
相关概念视频
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...