核糖体在拉切特的中间状态中的结构
Wen Zhang1, Jack A Dunkle, Jamie H D Cate
1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|August 22, 2009
概括
核糖体关涉及子单元旋转和头部域旋转. X射线结构揭示了中间状态,显示了核糖体子单元如何在蛋白质生物合成过程中重新排列接触.
科学领域:
- 分子生物学分子生物学
- 结构生物学是结构生物学.
- 生物化学 生物化学
背景情况:
- 蛋白质生物合成依赖于核糖体,这个过程涉及子单元旋转和头域旋转.
- 准确的分子机制,规范跨子单元的接触重排在拉切的过程中仍然不清楚.
研究的目的:
- 为了阐明核糖体子单元在拉切时重新排列的分子基础.
- 为了可视化Escherichia coli核糖体内的子单元间旋转的中间状态.
主要方法:
- 采用X射线晶体学来确定大肠杆菌核糖体的结构.
- 对于分别在3.5和4.0安格斯特姆的分辨率下模仿的apo-form和与结合的抗杆茎环tRNA的结构得到了获得.
主要成果:
- 这项研究揭示了沿着核糖体分离路径跨子单元旋转的离散中间状态.
- 小和大核糖体子单元之间的接口经历着逐步的重排.
- 小子单元的头域定位受到与大子单元和绑定tRNA的相互作用的影响.
结论:
- 观察到的结构中间体提供了对核糖体的动态过程的洞察.
- 这些发现揭示了tRNA进入混合状态的机制,先于mRNA和tRNA转位.
相关概念视频
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
01:27Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
01:27Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
01:27Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
01:27Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...