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相关概念视频

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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相关实验视频

Updated: Jun 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

转换成复制DNA发生在与过程性因子的相互作用中.

Adam R Parks1, Zaoping Li, Qiaojuan Shi

  • 1Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.

Cell
|August 26, 2009
PubMed
概括
此摘要是机器生成的。

细菌的转子子Tn7使用蛋白TnsE来准复制DNA. TnsE与DNA复制机器相互作用,引导转换到活跃的复制部位.

更多相关视频

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

相关实验视频

Last Updated: Jun 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 微生物学 微生物学

背景情况:

  • 细菌的转子子Tn7表现出一种喜欢插入到活跃复制DNA的偏好.
  • 这种转位是由转位子编码的蛋白质TnsE介导的.

研究的目的:

  • 研究TnsE与宿主DNA复制机器之间的相互作用.
  • 阐明Tn7针对活跃复制DNA的机制.

主要方法:

  • 在体内和体外的生物化学测试来研究蛋白质-蛋白质相互作用.
  • 使用纯化的蛋白质和DNA重构TnsABC+E转化反应.
  • 与DNA复制相关的转换位点选择的分析.

主要成果:

  • TnsE在物理和功能上与DNA复制过程性因子相互作用.
  • 一个体外转换系统 (TnsABC+E) 已成功复制.
  • 过程性因子被证明可以重新排序TnsE介导的转换事件,反映体内复制偏差.

结论:

  • TnsE与复制机器的相互作用是Tn7选择目标地点的关键.
  • 这种机制使Tn7能够优先转化为活跃复制DNA.
  • 其他移动遗传元素也可能采用类似的机制.