miR156调节的SPL转录因子定义了Arabidopsis thaliana中的内源性开花路径
Jia-Wei Wang1, Benjamin Czech, Detlef Weigel
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany.
Cell
|August 26, 2009
概括
植物的开花时间由两个途径控制:FT/FD复合体和microRNA156向的SPL基因. 这些途径通过激活MADS盒子基因来促进生殖发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 发育生物学 发展生物学
背景情况:
- 开花时间是一个关键的发育过程,受到环境因素的影响,如白天的长度.
- 开花地点T (FT) 蛋白和开花时间BZIP (FD) 转录因子形成一个复合体,触发过渡到开花的过程.
- 微RNA在植物发育中起着调节作用,但它们在开花途径中的具体参与是复杂的.
研究的目的:
- 为了研究微RNA156向SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) 基因在开花控制中的作用.
- 为了确定FT/FD路径和miR156/SPL路径在调节开花时间中的关系.
- 阐明这些途径融合以促进生殖发育的分子机制.
主要方法:
- 分析与开花时间相关的基因表达模式.
- 研究FT/FD,miR156和SPL基因之间的调控相互作用.
- 在射击顶部确定FT/FD和SPL的下游目标.
主要成果:
- SPL基因在FT/FD的下游和作为单独内源性开花途径的一部分发挥作用.
- 在miR156水平的下降导致SPL水平的增加,创造了开花的允许环境.
- FT/FD和SPL聚焦在重叠的目标上,SPL直接激活MADS盒子基因.
结论:
- miR156/SPL模块独立于,但与FT/FD模块融合,以控制开花时间.
- 这两种途径冗余和前性地调节了促进花的MADS盒子基因的激活.
- 这项研究揭示了一个复杂的分子框架,集成了环境和内生信号来控制开花.
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