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在小鼠的Hox-1.1转基因表达后,椎椎的变异

M Kessel¹, R Balling, P Gruss

¹Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Federal Republic of Germany.

|April 20, 1990

概括

改变小鼠的Hox-1.1基因导致严重的面和脊椎形,包括额外的脊椎. 这表明Hox-1.1在早期胚胎发育和模式形成中起着至关重要的作用.

科学领域:

发育生物学发展生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

鼠本体基因对胚胎发育至关重要.
了解它们的功能需要进行遗传分析.
之前的研究表明,Hox-1.1功能增益突变体在面缺陷下是不可行的.

研究的目的:

研究霍克斯-1.1基因在哺乳动物发育中的功能作用.
为了描述由Hox-1.1功能获取突变引起的发育异常.
为了确定胚胎发生过程中Hox-1.1干扰的关键时期.

主要方法:

使用类β-actin促进剂生成具有Hox-1.1功能增益突变的转基因小鼠.
对严重受影响的转基因动物进行详细的表型分析.
发育缺陷与特定胚胎时间点的相关性.

主要成果:

转基因小鼠表现出严重的面异常,并且在出生后无法生存.
具体的发育不良包括基础头骨,大 atlas 和轴骨的缺陷.
在骨结处观察到一个代表额外的脊椎的前骨.
发育干扰可能发生在妊娠9日左右,与神经峰迁移和索米特分化相吻合.

结论:

霍克斯-1.1基因对于部和部区域的正常发育至关重要.
霍克斯-1.1中的功能获取突变破坏了关键的发育过程.
霍克斯-1.1可能在早期胚胎模式和体质生成中起到关键的调节作用.

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