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Updated: Jun 20, 2026

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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在红色光蛋白mKeima中激发状态质子转移

J Nathan Henderson1, Maire F Osborn, Nayden Koon

  • 1Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA.

Journal of the American Chemical Society
|August 28, 2009
PubMed
概括

mKeima是一种红色光蛋白,由于激发状态质子转移 (ESPT),它表现出很大的斯托克斯转移. 这项研究阐明了其独特的光谱特性和绿色辐射背后的分子机制.

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...