p185neu蛋白和EGF受体的分子间联结调节了EGF受体功能
1Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.
Cell
|June 29, 1990
概括
皮肤表皮生长因子受体 (EGFR) 和p185neu在接触EGF时形成异构体. 这种EGFR-p185neu复合体影响EGFR.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 在瘤学瘤学.
背景情况:
- 表皮生长因子受体 (EGFR) 和p185neu是关键的细胞表面受体,参与细胞生长和分化.
- 通过这些受体发出的异常信号与各种癌症有关.
研究的目的:
- 为了研究EGFR和p185neu之间的潜在异构化.
- 描述EGFR-p185neu异构化对EGFR信号传递和连接体结合的功能后果.
主要方法:
- 在共同表达EGFR和p185neu的细胞系上使用交联试剂.
- 用抗EGFR和抗p185neu抗体进行免疫沉.
- 对EGF进行Scatchard分析以确定EGFR的亲和状态.
主要成果:
- 观察到一种高分子量复合物,被确定为EGFR-p185neu异构体.
- 异种化是由表皮生长因子 (EGF) 暴露引起的.
- 对EGF,EGFR表现出三种亲和状态 (低,高和非常高).
- 具有非常高亲和度的EGFR细胞对低水平的EGF表现出了显著的反应.
- 用抗p185neu抗体的治疗降低了p185neu的调节,并导致了非常高亲和度EGFR的消失.
- 在表达一种或两种受体类型的细胞中,EGF和TPA对EGFR进行了差异调节.
结论:
- EGFR和p185neu可以形成异构体,这个过程取决于EGF.
- EGFR-p185neu异构化会影响EGFR对EGF的亲和力及其生物反应.
- 向p185neu可以调节EGFR活性,这表明这些受体共同表达的癌症中潜在的治疗策略.
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