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Updated: Jun 20, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

从Streptomyces thioluteus中获得的氨酸氧化酶,AurF中的一个长寿命的基质氧化过氧铁 (III/III) 中间体

Victoria Korneeva Korboukh1, Ning Li, Eric W Barr

  • 1Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Journal of the American Chemical Society
|September 8, 2009
PubMed
概括

氨基氧酶AurF酶使用稳定的过氧-Fe(2)(III/III) 中间体来氧化p-aminobenzoate (pABA). 这种中间体迅速氧化pABA,这表明它在完全转化为p-nitrobenzoate (pNBA) 中的作用.

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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