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相关概念视频

Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

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相关实验视频

Updated: Jun 20, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

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Published on: April 30, 2011

嵌合物非同线性转录的含义

Thomas R Gingeras1

  • 1Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA. gingeras@cshl.edu

Nature
|September 11, 2009
PubMed
概括
此摘要是机器生成的。

研究人员在从虫到人类的生物体中发现了非共线性转录,称为chimaeric RNAs. 这些转录是由遗传或转录后事件形成的,挑战了我们对基因组信息内容的理解.

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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
11:13

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries
07:35

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

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Published on: April 30, 2011

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.

背景情况:

  • 深度测序揭示了转录组,即完整的RNA转录组.
  • 一些转录表现出非共线性结构,具有来自遥远DNA区域的段落.

研究的目的:

  • 研究非同线性转录的起源和影响.
  • 了解在较高的真核生物中构成甲基RNA的机制.

主要方法:

  • 在各种物种中对转录组进行深度测序.
  • 对RNA序列组成和基因组起源的分析.

主要成果:

  • 识别了由非连续的DNA段组成的基因组转录.
  • 观察到由遗传重组和转录后事件引起的基因组RNA.
  • 突出了对更高的真核细胞中转接合的有限理解.

结论:

  • 化学RNA是整个动物王国的一个重要特征.
  • 这些发现扩大了已知的基因组信息及其排列的复杂性.
  • 需要进一步的研究,以阐明在更高的真核生物中基马基RNA的形成.