Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evidence that zymocin-like killer plasmids were present in the common ancestor of terrestrial fungi.

Genome biology and evolution·2026
Same author

The molecular determinants of PABPC-mediated deadenylation rate.

bioRxiv : the preprint server for biology·2026
Same author

Global stabilization of the transcriptome in mitotic cells.

The EMBO journal·2026
Same author

Rapid and repeated evolution of myosin copy number in threespine stickleback.

Current biology : CB·2026
Same author

mRNA 3' UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth.

Genes & development·2026
Same author

The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation.

Nature·2026
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

在芽酵母中的RNAi.

Ines A Drinnenberg1,2, David E Weinberg1,2,3, Kathleen T Xie1,2,3

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|September 12, 2009
PubMed
概括
此摘要是机器生成的。

在像Saccharomyces castellii这样的芽酵母中存在RNA干扰 (RNAi),使用新的Dicer蛋白. 在Saccharomyces cerevisiae中重建RNAi使逆转移子沉默,提供了新的研究工具.

更多相关视频

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

相关实验视频

Last Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 酵母生物学的酵母生物学

背景情况:

  • RNA干扰 (RNAi) 是一种在真核生物中保存的基因沉默机制.
  • 在模型生物Saccharomyces cerevisiae中,RNAi途径显著缺席.
  • 之前的研究表明,某些芽的酵母物种中RNAi的损失.

研究的目的:

  • 为了研究RNAi在Saccharomyces cerevisiae之外的发芽酵母物种中的存在和机制.
  • 为了确定这些物种中涉及RNAi的特定蛋白质.
  • 探索在Saccharomyces cerevisiae中重建RNAi的潜力,以研究基因沉默.

主要方法:

  • 对Saccharomyces castellii和Candida albicans中的RNAi通路组件进行比较基因组学和分子分析.
  • 非正规的Dicer蛋白的识别和表征.
  • 功能性测试用于评估RNAi活性和目标基因沉默.
  • 对Saccharomyces cerevisiae进行基因操纵,以引入和测试来自S. castellii的RNAi组件.

主要成果:

  • 在Saccharomyces castellii和Candida albicans中检测到RNAi,使用非正规的Dicer蛋白.
  • 在这些物种中产生的小干扰RNA主要向可转移元素和Y'亚端粒重复.
  • 在S. castellii中缺少RNAi的突变体表现出较高的Y'信使RNA水平.
  • 在Saccharomyces cerevisiae中引入S. castelliiDicer和Argonaute恢复了RNAi,导致了内源逆转移子的沉默.

结论:

  • 在芽酵母中发现了一种在RNAi中功能性的Dicer蛋白的新型类型.
  • 这项研究重新引入了RNAi工具,用于研究在芽酵母中的基因沉默.
  • 这项研究使芽酵母系统的应用能够研究RNAi机制.