外体调节昼夜基因表达在一个转录后的负反循环中
Jinhu Guo1, Ping Cheng, Haiyan Yuan
1Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Cell
|September 15, 2009
概括
该FFC复合体通过控制frqRNA衰变通过外体调节昼夜节律. 这种转录后反循环确保了时钟基因表达的稳健节奏性.
科学领域:
- 分子生物学分子生物学
- 循环节生物学 循环节生物学
- 基因规则 基因规则
背景情况:
- 细胞的昼夜振荡器依赖于负反循环.
- 这些时钟中转录后RNA调节的作用在很大程度上是未知的.
- 在神经菌中,FFC复合体抑制了frq转录.
研究的目的:
- 为了研究转录后调节在神经的昼夜钟中的作用.
- 为了确定FFC复合体是否影响RNA稳定性.
- 阐明控制frqRNA节律性的机制.
主要方法:
- RNA免疫沉 (RIP) 来检测FFC-RNA相互作用.
- 通过沉默RRP44.4进行外体细胞功能测试.
- 在不同条件下分析frqRNA和蛋白质水平.
- 奇普测试以评估白领复合体与rrp44的结合.
主要成果:
- FFC与frqRNA结合并与外体相互作用,促进frqRNA的衰变.
- 当FRQ水平较低时,FRQRNA表现出强大的节奏性,稳定性更高.
- 沉默RRP44会增加frqRNA水平,并破坏时钟功能.
- rrp44是一种时钟控制的基因,是白领复合体的直接目标,调节其他时钟控制的基因.
结论:
- FFC和外体形成一个转录后负反循环,调节frqRNA水平.
- 这种机制对于保持强大的昼夜节律和时钟输出至关重要.
- 转录后控制在真核生物昼夜振荡器中起着重要作用.
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