通过使用可切换光的蛋白相互作用来控制细胞信号的时空控制
Anselm Levskaya1, Orion D Weiner, Wendell A Lim
1The Cell Propulsion Lab, UCSF/UCB NIH Nanomedicine Development Center, University of California, San Francisco, California 94158-2517, USA.
Nature
|September 15, 2009
概括
科学家们设计了一种新的光控制系统,利用植物蛋白精确操纵细胞行为. 这种基因编码的系统允许可逆的蛋白质转位,为细胞生物学研究提供了新的工具.
科学领域:
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
- 分子生物学分子生物学
背景情况:
- 像GFP这样的基因编码的光学记者能够进行细胞观测.
- 细胞行为的光学控制是具有挑战性的,因为工程光敏感蛋白质的困难.
- 现有的光学控制方法包括半合成受体和通道罗多素,主要用于神经网络.
研究的目的:
- 开发一种新的,基因编码的系统,用于精确的光学控制细胞功能.
- 为可逆细胞操纵设计一种光敏感的蛋白质-蛋白质相互作用.
- 为了证明该系统在控制蛋白质定位和细胞形态方面的实用性.
主要方法:
- 适应和优化了来自Arabidopsis thaliana植物染色体信号网络的可逆蛋白-蛋白相互作用.
- 基于这种相互作用,设计了一种基因编码的光控制系统.
- 利用该系统实现蛋白质向细胞膜的光门转移.
- 应用该系统来控制Rho-家族GTPase激活剂以影响actin细胞骨架和细胞形态.
主要成果:
- 通过空间和时间分辨率 (微米,秒) 证明了目标蛋白质到膜的精确和可逆转移.
- 展示了Rho-家族GTPase激活剂的光门转位.
- 通过光诱导的细胞骨变化,成功地重塑和指导哺乳动物细胞形态.
- 验证了该系统用于控制各种细胞功能的通用适用性.
结论:
- 开发的光门蛋白-蛋白相互作用系统为细胞生物学中的光学控制提供了一个强大的新工具.
- 该系统能够精确,可逆地操纵高空间和时间分辨率的细胞过程.
- 它具有创造光可编程试剂和推进扰动性,定量细胞生物学实验的潜力.
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