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Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
Published on: June 16, 2011
热冲击蛋白65作为胰岛素依赖糖尿病的β细胞抗原
D B Jones1, N R Hunter, G W Duff
1Department of Medicine, Western General Hospital, Edinburgh, UK.
Lancet (London, England)
|September 8, 1990
概括
来自Mycobacterium tuberculosis的热冲击蛋白65 (HSP65) 可能是胰岛素依赖糖尿病的主要β细胞抗原. 对HSP65的自身免疫可能会增加对这种自身免疫性疾病的易感性.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 微生物学 微生物学
背景情况:
- 在胰岛素依赖糖尿病中,主要的β细胞抗原是~64kD的蛋白质.
- 胰岛素瘤细胞在受到高热和细胞因子 (如白蛋白-1β,胺干扰素和瘤亡因子) 的刺激时合成这种64kD蛋白.
研究的目的:
- 为了调查64kDβ细胞抗原的身份.
- 探索热冲击蛋白在胰岛素依赖糖尿病病原发生中的潜在作用.
主要方法:
- 西部斑点分析用于评估64kDβ细胞蛋白与来自Mycobacterium tuberculosis和Mycobacterium leprae的热冲击蛋白65 (HSP65) 的抗体之间的交叉反应性.
- 用胰岛素依赖糖尿病患者的血清进行了抑制试验.
主要成果:
- 在64kD的β细胞蛋白和针对Mycobacterium tuberculosis HSP65的抗体之间观察到交叉反应,但不是针对Mycobacterium leprae HSP65.
- 来自胰岛素依赖的糖尿病患者的血清含有64kDβ细胞抗原的抗体,抑制了Mycobacterium结核病HSP65抗体与细胞因子治疗细胞的结合.
结论:
- 热冲击蛋白65 (HSP65) 被认为是64kD的β细胞抗原.
- 对HSP65的一个表位素的自身活性可能会使人对胰岛素依赖的糖尿病产生敏感性.
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