有证据表明,染色体17p上的至少两个基因与乳腺癌发生有关
C Coles1, A M Thompson, P A Elder
1Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Lancet (London, England)
|September 29, 1990
概括
研究人员在乳腺癌患者的染色体17上绘制了异构性损失的地图. 在17p13-3上的p53基因附近的一个调节基因与大多数乳腺癌有关.
科学领域:
- 遗传学 是一个遗传学.
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 在各种人类癌症中经常观察到染色体17上的异构性 (LOH) 丧失.
- 位于染色体17p13.1上的p53基因是一个关键的瘤抑制基因.
- 之前的研究表明,染色体17与乳腺癌的发展有关.
研究的目的:
- 在大量乳腺癌患者群体中识别和绘制17号染色体上的LOH区域.
- 研究17号染色体特定位置的LOH与乳腺癌之间的关系.
- 探索一个调节基因对p53的端粒在乳腺癌发病过程中的潜在作用.
主要方法:
- 来自乳腺癌患者的瘤和血液白细胞样本的DNA分析.
- 微卫星分析以检测第17号染色体上的异构性损失.
- 对p53 mRNA表达的定量分析.
主要成果:
- 在染色体17p上证实了LOH的高频率.
- 三分之一的信息性瘤在长臂THH59位点表现出异基损失.
- 在短臂上确定了两个不同的LOH区域:17p13-3和17p13-1 (可能涉及p53).
- 与17p13-1相比,17p13-3区域的LOH频率明显高,而17p13-1区域的LOH频率明显高.
- 在两个位点的等位基因损失之间没有观察到相关性.
- 在17p13-3的LOH与p53mRNA过度表达有关.
结论:
- 17p13-3的LOH在乳腺癌中很常见,并且与p53 mRNA过度表达有关.
- 在17p13-3上,一个约为20兆基的调节基因与p53的端粒似乎与乳腺癌有关.
- 这种调节基因的损伤可能在大多数乳腺癌病例中发挥重要作用.
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