Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 20, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

微流体装置用于单分子实验,增强光稳定性.

Edward A Lemke1, Yann Gambin, Virginia Vandelinder

  • 1Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Journal of the American Chemical Society
|September 24, 2009
PubMed
概括

这项研究引入了一种聚甲基西洛 (PDMS) 微流体装置,可以增强单分子光实验. 该设备实现了高染料光稳定性,并通过脱氧和快速缓冲区交换减少了样品粘附.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Bis-Tetrazine Fluorogenic (Silicon)-Rhodamine Dyes for Live-Cell Labeling.

Journal of the American Chemical Society·2026
Same author

The filamentous ultrastructure of the PopZ condensate is required for its cellular function.

Nature structural & molecular biology·2026
Same author

Direct RNA sequencing enables improved transcriptome assessment and tracking of RNA modifications for medical applications.

Nucleic acids research·2025
Same author

Biomolecular Condensates Can Induce Local Membrane Potentials.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases.

Nature communications·2025
Same author

Spheres, worms, and packing problems in the nanoscale assembly of ALS-linked protein Matrin-3.

Molecular cell·2025

科学领域:

  • 生物物理学的生物物理.
  • 微流体学 微流体学
  • 分析化学 分析化学

背景情况:

  • 单分子光实验面临着诸如染料光漂白和样品吸附等局限性.
  • 现有的方法很难长时间保持最佳的实验条件.

研究的目的:

  • 开发一种微流体装置,克服单分子光研究中的关键局限性.
  • 为了提高染料的光稳定性,并最大限度地减少非特异性样品结合.

主要方法:

  • 一种具有多孔通道壁的聚甲基素 (PDMS) 微流体装置的制造.
  • 通过气体扩散实施脱氧化策略,以降低氧气水平.
  • 采用层状流来实现快速的缓冲区交换和最小的表面样本相互作用.

主要成果:

  • 实现显著减少光染料的光漂白.
  • 证明样品对微流体通道的粘附率较低.
  • 能够在现场添加和组合试剂进行动态实验.

结论:

  • PDMS微流体装置为先进的单分子光实验提供了一个强大的平台.
  • 集成的脱氧和快速交换系统提高了数据质量和实验灵活性.

更多相关视频

A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

相关实验视频

Last Updated: Jun 20, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009