一种用于花粉发育的新路径的进化
Michiyo Matsuno1, Vincent Compagnon, Guillaume A Schoch
1Institut de Biologie Moléculaire des Plantes, CNRS-UPR2357, Université de Strasbourg, 28 Rue Goethe, 67083 Strasbourg Cedex, France.
概括
植物进化涉及基因的创造适应. 在Brassicaceae中,一种新的路由通过基因重复和细胞染色体P450酶的修改进化,影响了甲代谢.
科学领域:
- 植物生物学 植物生物学
- 分子进化的分子进化.
- 生物化学 生物化学
背景情况:
- 由基因创造驱动的代谢可塑性对于植物的适应和物种化至关重要.
- 细胞染色体P450酶的多样化说明了植物中的这一进化过程.
研究的目的:
- 阐明导致Brassicaceae中新型性途径的进化途径.
- 了解基因复制和新功能化在酶进化中的作用.
主要方法:
- 对逆位,新功能化和基因重复事件的分析.
- 在细胞P450酶中选择性氨基酸替代物的研究.
- 对N1,N5-di(hydroxyferuloyl) -N10-sinapoylspermidine合成的六基化途径的表征.
主要成果:
- 在Brassicaceae中通过反置,新功能化和重复的序列进化了一个新的路径.
- 两个部分冗余的P450酶催化了六次连续的氧化.
- 该途径产生N1,N5-di(基) -N10-sinapoylspermidine,这是一个关键的花粉成分.
结论:
- 积极的达尔文选择通过有针对性的氨基酸替代驱动酶进化.
- 这项研究揭示了以前被忽视的烯胺代谢的途径.
- 基因重复和新功能化是植物代谢创新的关键机制.
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