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相关概念视频

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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相关实验视频

Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

条件RNA干扰由全性 рибо酶介导.

Deepak Kumar1, Chung-Il An, Yohei Yokobayashi

  • 1University of California, Davis, Department of Biomedical Engineering 451 Health Sciences Drive, Davis, California 95616, USA.

Journal of the American Chemical Society
|October 1, 2009
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的RNA系统,用于精确控制哺乳动物细胞中的基因沉默. 这种化学诱导RNA干扰 (RNAi) 方法为调节基因表达提供了模块化和可适应的方法.

更多相关视频

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

相关实验视频

Last Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 基因规则 基因规则

背景情况:

  • 有条件的RNA干扰 (RNAi) 允许控制的基因沉默.
  • 现有的方法通常需要复杂的基因修改,限制了它们的应用.
  • 需要更简单,化学诱导的RNAi系统.

研究的目的:

  • 设计一种新的RNA架构,用于哺乳动物细胞中化学诱导RNAi.
  • 为条件基因沉默创建一个紧和模块化系统.
  • 为了使RNAi系统能够响应各种分子信号.

主要方法:

  • 设计了一种新的RNA结构,结合了可诱导药物的全性 рибо酶和微RNA前体类似物.
  • 在哺乳动物细胞中利用化学诱导来激活RNAi.
  • 证明了RNA设计的模块化性,以实现潜在的适应.

主要成果:

  • 在哺乳动物细胞中成功开发出一种可化学诱导的RNAi系统.
  • 新的RNA架构提供了一个紧而模块化的设计.
  • 该系统能够通过RNA体对各种分子进行感知和响应.

结论:

  • 新的RNA设计能够对基因沉默进行精确的化学控制.
  • 这种模块化系统有助于构建多功能条件RNAi应用程序.
  • 这种方法为研究和潜在的治疗中调节基因表达提供了一个有希望的工具.