相关实验视频
Updated: Aug 8, 2026
07:40
Dissection and Staining of Drosophila Larval Ovaries
Published on: May 13, 2011
在Drosophila中对同源基因控制的目标
A P Gould1, J J Brookman, D I Strutt
1Department of Anatomy, University of Cambridge, UK.
Nature
|November 22, 1990
概括
研究人员在Drosophila胚胎中确定了Ultrabithorax (Ubx) 蛋白质结合部位. 这些位点靠近由Ubx调节的基因,这表明它们是控制细分发育的基因.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 家庭基因Ultrabithorax (Ubx) 对于确定Drosophila的细分身份至关重要.
- Ubx编码的转录因子与家庭主体,调节在发育过程中基因表达.
研究的目的:
- 描述一种方法来分离由Ubx蛋白结合的胚胎染色质碎片.
- 为了识别Drosophila胚胎中的Ubx潜在的直接标基因.
主要方法:
- 胚胎染色质碎片含有Ubx结合点的免疫净化.
- 对已知转录单元相对鉴定Ubx结合位点的基因组位置的分析.
主要成果:
- 一个免疫净化程序成功丰富了Ubx结合的染色素.
- 在两个实例中,Ubx结合点被发现靠近胚胎转录单元.
- 这些转录单元在体内可能被Ubx位所调节.
结论:
- 描述的方法有效地识别了胚胎基因组中的Ubx结合位.
- 已识别的Ubx结合位点位于潜在目标基因附近.
- 这些发现表明,新的Ubx目标基因参与了细分特定发育途径的指定.
相关概念视频
02:32Position-effect Variegation
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
02:45The Ratio of X Chromosome to Autosomes
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...