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发现了Atg5/Atg7独立的替代性宏自的发现
Yuya Nishida1, Satoko Arakawa, Kenji Fujitani
1Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Nature
|October 2, 2009
概括
哺乳动物的宏观自,一种细胞降解过程,可以通过两个途径发生. 另一种替代的,Atg5/Atg7独立的通路存在于常规通路旁边,对某些细胞功能至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 自学研究 自学研究
背景情况:
- 宏自是一种基本的细胞过程,通过自细胞/自解细胞通过自细胞/自解细胞来降解受损成分.
- Atg5和Atg7被广泛认为是哺乳动物宏观自开始和执行的基本基因.
研究的目的:
- 调查在没有Atg5或Atg7.7的情况下发生的宏自的存在和特征.
- 为了确定自细胞形成和降解的替代机制.
主要方法:
- 使用过的小鼠细胞缺乏Atg5或Atg7.
- 在压力条件下分析了自细胞/自解细胞的形成和蛋白质降解.
- 研究了与自相关的特定蛋白质 (Ulk1,beclin 1) 和Rab9.9) 的作用.
- 在体内胚胎组织中检查了宏观自.
主要成果:
- 缺乏Atg5或Atg7的小鼠细胞仍然可以进行宏自和降解蛋白质.
- 在这种替代途径中,自细胞的形成独立于LC3脂化 (LC3-II).
- 独立于Atg5/Atg7的途径涉及Ulk1,贝克林1和Rab9-依赖的囊泡融合来自跨戈尔吉和晚期内分泌体.
- 这种替代途径在胚胎组织中观察到,并且在红细胞成熟期间参与线粒体清除.
结论:
- 哺乳动物的宏观自至少通过两条不同的路径进行:传统的Atg5/Atg7依赖路径和替代的Atg5/Atg7独立路径.
- 发现一种替代途径扩大了我们对细胞降解机制及其调节的理解.
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