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鼠标Hox-2.2指定了Drosophila胚胎和幼虫中的胸部细分标识

J Malicki¹, K Schughart, W McGinnis

¹Department of Human Genetics, Yale University, New Haven, Connecticut 06511.

|November 30, 1990

概括

鼠标的Hox-2.2基因可以替代Drosophila Antennapedia基因,在发展中果中诱导类似的同源转化. 这表明胚胎发育和基因调节中的功能得到保护.

科学领域:

发展生物学发展生物学
遗传学是一个遗传学.
进化生物学进化生物学

背景情况:

鼠标基因组中含有同源盒基因,同类于Drosophila Antennapedia (Antp) 基因.
家庭盒子基因在胚胎发育和建立身体规划模式中起着至关重要的作用.

研究的目的:

为了研究小鼠Antp样基因 (Hox-2.2) 和DrosophilaAntp基因之间的功能保护.
确定小鼠Hox-2.2是否可以在Drosophila发育过程中功能性地替代Antp.

主要方法:

鼠标Hox-2.2在使用热冲击促进剂发育的多索菲拉细胞中的表达.
在幼虫和成年Drosophila中诱导的同源转换的分析.
对Hox-2.2与内源性Antp基因之间的相互作用的评估.

主要成果:

鼠标Hox-2.2的子宫外表达诱导了Drosophila的同源性转变,模仿了Antp.引起的变化.
转变包括幼虫牙带和成人的附属物 (腿取代天线) 的变化.
霍克斯-2.2的表型效应独立于内源性Antp基因的表达.

结论:

鼠标Hox-2.2可以在功能上替代Drosophila Antp,以指定细分身份.
这表明保存下游基因调节和进化保存homeobox基因功能.
霍克斯-2.2可能会调节与Drosophila胚胎中的Antp相同的下游目标.

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